Finally, all of this conversation fails to take into account that the Cov 2 virus itself should have been isolated long ago if it were actually real. There would be no need to guess at its genome from microscopic material that could come from a variety of things. Virus Mania gets into this:
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Lack of Detection of So-Called SARS-CoV-2
Incidentally, the virus hunters have conveniently ignored a pivotal scientific principle in their argument. Complete purification is an indispensable pre-requisite for virus identification as stated by textbooks1350 1351, virus researchers such as Luc Montagnier (see box with quotes from well-known experts) and the second of Koch’s postulates (see chapter 3, subchapter “Where Is the Proof of HIV?”).
“Purification”, mind you, means the separation of an object from everything that does not belong to it—as, for example, Nobel Prize winner Marie Curies isolated radium from tons of pitchblende in 1898. Only on the basis of such a complete purification can it be proven that the nucleic acid sequences found in the particles in question originate from a new virus.
For this, one must remember that the PCR is extremely sensitive. This means that it can “pick up” even the smallest genetic fragments—i.e. DNA or RNA fragments. But it is not possible with the PCR to determine where these nucleic acid sequences come from. This must be determined beforehand in a separate process. And since PCR tests are “calibrated” to nucleic acid sequences, in this case RNA sequences (since it is assumed that SARS-CoV-2 is an RNA virus), it must of course be clearly proven that these genetic fragments are actually part of the claimed virus. And in order to prove this beyond any doubt, the correct isolation and complete purification of the suspected virus are indispensable pre-requisites.
To make this quite clear once again, it is worthwhile to employ a paternity suit analogy. Here, in order to compare the DNA of the suspected father and the child, one must ensure that the DNA is extracted from the bodies of the alleged father and the child. The same standard undoubtedly applies to determining whether RNA belongs to a virus or not. In a paternity suit, the genome can, mind you, be extracted from a single “particle” (father/ child). This is different for particles suspected of being viruses. The viral genome cannot be obtained from a single particle due to its extremely small size. This means that it must be obtained from a large mass of identical, i.e. completely purified particles, or at least from material that does not contain any foreign RNA.
Thus, when cells, cell debris and particles are mixed in a laboratory culture, the only way to determine which RNA (or even proteins) are viral is to separate the particles from all non-viral material. However, some researchers use the term “isolation” in their work to give the impression to the uninitiated reader that a virus has been isolated in pure form. In fact, however, this has not happened, because the procedures described in these works do not represent a proper process of isolation including complete purification. Consequently, they misuse the term “isolation” in their publications.
And so we decided to be the first in the world to ask the research teams of the relevant papers cited in connection with the alleged detection of SARS-CoV-2 whether the electron microscope images shown in their in vitro studies depict completely purified viruses. However, not a single team of authors—including those of two pivotal studies (Zhu et al., Wan Beom Park et al. )—could answer this question with a yes. And it should be noted that no one wrote back suggesting that complete purification is not a necessary step for solid virus detection.
We also contacted Charles Calisher, who is a seasoned virologist. In 2001, Science published an “impassioned plea … to the younger generation” from several veteran virologists, among them Calisher, saying that “[modern virus detection methods like] sleek polymerase chain reaction … tell little or nothing about how a virus multiplies, which animals carry it, [or] how it makes people sick. [It is] like trying to say whether somebody has bad breath by looking at his fingerprint.”1355 And that’s why we asked Calisher whether he knows of a single paper in which SARS-CoV-2 had been isolated and then truly purified. His answer: “I know of no such a publication. I have kept an eye out for one.”1356
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Source: Engelbrecht, Torsten; Köhnlein, Claus; Bailey, Samantha; Scoglio, Stefano. Virus Mania (pp. 387-389). Books on Demand. Kindle Edition.
The book goes on, talking about various efforts to find a purified Cov 2 virus. So far, nothing.