I did make that assertion, although I think that Dr. Mark Bailey gives a better explanation of what's going on. I've quoted his explanation in
Post #532.
Nowhere in the quote does Bailey address the actual process of how de novo assembly works.
If you're interested in the definition (or at least -a- definition) of de novo assembly, I recommend the following article:
What is de novo assembly? |*thesequencingcenter.com
The article makes it clear from the outset that there is some inconsistency when it comes to the definition, so it sets out its own definition. If we can agree with that definition for the purposes of this discussion, I think we should be good.
He doesn't discuss the computer code. He doesn't discuss the overlapping segments.
He actually does mention hypothetical overlapping segments in the construction of the Cov 2 virus, I just didn't quote that part. I'll quote the paragraph where he mentions it, as well as the 2 subsequent paragraphs. I've bolded 2 contiguous sentences that I think are highly important- the first mentions how their remained a high match for known human RNA sequences and the second talks about the precise de novo assembly method and software used, which is something you seemed interested in as well...
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In any case, they obtained some bronchoalveolar lavage fluid (BALF) from their patient and with this crude specimen reported that, “total RNA was extracted from 200μl of BALF.” Their methods section detailed that this was achieved, “using the RNeasy Plus Universal Mini kit (Qiagen),” i.e. through spin column centrifugation.
They claimed that, “ribosomal RNA depletion was performed during library construction,” however, see page 43 as to why this is dubious as there remained a high match for known human RNA sequences. They then proceeded to shotgun sequence the brew, starting with random fragmentation of the genetic material into short lengths averaging 150 nucleotides and conversion of the RNA to DNA using a reverse transcriptase enzyme.90 56,565,928 such short reads were generated and this information was fed into Megahit and Trinity, software platforms for de novo algorithm-based assembly. Through Megahit, 384,096 contigs, or hypothetical overlapping sequences were generated and the longest one (30,474 nucleotides) was declared to have a “nucleotide identity of 89.1%” to bat SL-CoVZC45, another fictional construct that will be dealt with subsequently. (Trinity generated over 1.3 million contigs but the longest one was only 11,760 nucleotides — in other words, they would not have found the “genome” if they had just used this software platform.) The word ‘virus’ suddenly appeared when they state, “the genome sequence of this virus, as well as its termini, were determined and confirmed by reverse- transcription PCR.” This is a sleight of hand as the PCR simply amplifies pre-selected sequences and has no capacity to confirm a previously unknown genome. As PCR expert Stephen Bustin has explained, “PCR requires you to know what the sequence of your target is...so once you know that there’s something in your sample, then you would try to isolate it, yes. And then once you’ve isolated it, then you sequence it again, or PCR it up.”91 In other words, PCR itself cannot identify the origins of the sequences and the methodology of Fan Wu et al. did not establish the origin of their described sequences. However, in the very next sentence they announce to the world that, “this virus strain was designated as WH-Human 1 coronavirus (WHCV)”.
— We need to pause at this point as it is where the fraudulent virus, soon to be renamed SARS-CoV-2, was invented out of thin air. A virus that the WHO claims, with no eviden6al support whatsoever, is the causa6ve agent of COVID-19.
For it is this “genome” that was submiged to GenBank on the 5th of January 202092 that was seized on by Drosten et al. to help produce their phoney PCR protocol assay sequences,93 which in turn were published with indecent haste by the WHO for all the world to use, thereby turning WH- Human 1 into the world’s reference genome for a claimed pathogen. It is this invenEon that is responsible for the whole bag of destrucEve tricks imposed on the world following the announcement of the pandemic by the WHO on the 11th of March 2020.94
However, anyone paying attention can see that there is no evidence whatsoever of a virus in the Fan Wu et al. paper. A virus is claimed to be a tiny replication-competent obligate intracellular parasite, consisting of a genome surrounded by a proteinaceous coat: it is an infectious particle that causes disease in a host. All Fan Wu et al. had was a 41-year-old man with pneumonia and a software-assembled model “genome” made from sequences of unestablished origin found in the man’s lung washings. To make it appear legitimate they stated, “the viral genome organization of WHCV was determined by sequence alignment to two representative members of the genus Betacoronavirus: a coronavirus associated with humans (SARS-CoV Tor2, GenBank accession number AY274119) and a coronavirus associated with bats (bat SL-CoVZC45, GenBank accession number MG772933).” These alleged genomes are also simply in silico constructs that have never been proven to exist in their entirety in nature, let alone been shown to come from inside a virus. For example bat SL-CoVZC45 was invented in 2018 by the process of, “19 degenerated PCR primer pairs...designed by multiple alignment of available SARS-CoV and bat SL-CoV sequences deposited in GenBank.”95
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Source:
A Farewell To Virology (Expert Edition) | drsambailey.com